Catalogue number
PRO-2217
Synonyms
BolA Family Member 3, MMDS2, BolA Homolog 3 (E. Coli), BolA-Like
3 (E. Coli), BolA-Like Protein 3, BolA Homolog 3, BolA-like protein 3.
Introduction
BolA Homolog 3, also known as BOLA3 is a member of the BolA/IbaG family.
BOLA3 is novel non-classical secreted protein which his secretion is non
dependent on its predicted signal peptide.
Furthermore, Human BolAs belongs to three different groups with
functional divergence of BolA subfamily, where the different helix-turn-helix
motif among BOLA 1, BOLA 2, as well as BOLA3 is responsible for their
functional divergence.
Description
BOLA3 Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 130 amino acids (1-107 a.a) and
having a molecular mass of 14.5kDa.
BOLA3 is fused to a 23
amino acid His-tag at N-terminus & purified by proprietary chromatographic
techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered colorless solution.
Formulation
BOLA3 protein solution (1mg/ml) containing Phosphate
buffered saline (pH7.4) and 10% glycerol.
Stability
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Avoid multiple freeze-thaw cycles.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Amino acid sequence
MGSSHHHHHH SSGLVPRGSH MGSMAAWSPA AAAPLLRGIR GLPLHHRMFA TQTEGELRVT QILKEKFPRA
TAIKVTDISG GCGAMYEIKI ESEEFKEKRT VQQHQMVNQA LKEEIKEMHG LRIFTSVPKR.
Safety Data Sheet
Usage
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
