CHOPWT9 iPSC |产品详情|进口榴莲视频免费观看采购网

您好，欢迎来到榴莲视频下载地址入口生物网站！请登录注册

我的订单 | 联系榴莲视频下载地址入口 | 在线客服QQ：3012681483 | 客服电话：400-968-7988 | 工作时间：08:30-17:30

主营产品： ELISA8008APP幸福宝榴莲、抗体、补体血清、兔补体、干扰素、小鼠测温仪、进口榴莲视频免费观看、Quidel代理、Swant代理、PBL Assay Science代理

网站首页产品中心代理品牌新闻资讯资源中心关于榴莲视频下载地址入口

所在位置：首页 > 产品中心 > abmgood > abmgood产品

订购信息

上海榴莲视频下载地址入口生物科技公司

Tel:400-968-7988 021-33779008

CHOPWT9 iPSC

品牌：Abmgood

货号：

规格：

货期：

应用：

咨询客服

立即购买加入购物车

CHOPWT9 iPSC

商品详情参考文献相关资料

	Print Version
Safety	Biosesafety Level II
Organism	Human
Donor	Female
Source Organ	Peripheral Blood Mononuclear Cells
Growth Properties	Adherent
Morphology	Polygonal
Markers	Oct4, Sox2, Nanog, Rex1, DMNT3B, and ABCG2
Picture
Description	The CHOPWT9 IPSCs were established by Sendai viral infection carrying reprogramming factors Oct3/4, Sox2, c-Myc, and Klf-4 of peripheral blood mononuclear cells collected from a healthy ***** female. These cells are capable of in vivo tetratoma formation as tested on NOD/SCID mice. The CHOPWT9 IPSCs are valuable in the field of developmental biology as they are useful as a control for applications such as differentiation analyses to the three germ layers and derivative tissues.
Quality Control	1) Flow cytometry analysis of pluripotency markers; 2) Karyotype analysis of chromosomal G-band; 3) qPCR of pluripotency genes
Concentration	10⁶cells/vial
Propagation	The iPSCs requires a co-culture with mouse embryonic fibroblasts (MEFs) to proliferate. The base medium for this cell line is Prigrow IV medium available from abm (TM004). To make the completed growth medium, add the following components to the base medium: KnockOut^TM Serum Replacement (Invitrogen; A3181502) to final concentration of 15%, 1% NEAA (Invitrogen; 11140050), 1% L-glutamine (G275), 0.1 mM 2-mercaptoethanol (Invitrogen; 21103-049) and 1% Penicillin/Streptomycin Solution (G255). Filter medium with 0.45 µM, before adding 5-10 ng/ml human bFGF (Z101455).Atmosphere: air: 95%, CO₂: 5%; Temperature: 37.0°C. Add 10 µM ROCK inhibitor (R&D Systems; 1254/50) to media when plating iPSCs on MEFs.
Storage	-180°C
Shipping	On Dry Ice
Notes	For Research Use Only, not for therapeutic or diagnostic purposes.