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CHAPS, High Purity

品牌：AG Scientific

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规格：5 g

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应用：

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CAS:75621-03-3Formula:C32H58N2O7SMW:614.9Appearance:WhiteSolidPurity:98%

商品详情参考文献相关资料

CHAPS is a zwitterionic surfactant derived from cholic acid, primarily used to solubilize membrane proteins and other macromolecules. Can be easily removed from gels or protein solutions by dialysis across a membrane. It combines the features of bile salts and N-alkyl sulfobetaine

CHAPS is used as a nondenaturing solvent in the process of protein purification and is especially useful in purifying membrane proteins, which are often sparingly soluble or insoluble in aqueous solution due to their natively hydrophobic cellular environment. When in salt, CHAPS tends to behave as a weakly charged cation. However, in water or buffer, it behaves as a nonionic surfactant.

CHAPS detergents have low light absorbance in the ultraviolet region of the electromagnetic spectrum, which is useful for laboratory workers monitoring ongoing chemical reactions or protein-protein binding with UV/VIS spectroscopy. CHAPS is also useful in ion exchange chromatography and isoelectric focusing as it is zwitterionic and does not exhibit a net charge between pH2 to12. The critical micelle concentration of CHAPS is 6-10mM.

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Additional Information

Synonyms	3-[3-(Cholamidopropyl)dimethylammonio]-1-proanesulfonate
Product #	C-1019
CAS #	75621-03-3
Chemical Name	3-[3-(Cholamidopropyl)dimethylammonio]-1-propanesulfonate
Formula	C₃₂H₅₈N₂O₇S
MW	614.9
Appearance	White Solid
Purity	98%
Solubility	Soluble in DMSO or DMF.
Melting Point	157 °C
Storage Temp	Room Temp
Use	A non-denaturing, zwitterionic detergent for membrane proteins

Additional Information

Merck Index	Merck Index: 13.2052.2001
MDL Number	MFCD00012116
ChemACX	X1016609-1
InChI	InChI=1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
SMILES	C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS(=O)(=O)[O-])[C@H]1CC[C@@H]2[C@@]1([C@H](C[C@H]3[C@H]2[C@@H](C[C@H]4[C@@]3(CC[C@H](C4)O)C)O)O)C