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Ready-to-use fluorometric substrate for caspases that recognize the amino acid sequence ATAD. Caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate ATAD-AFC at Ex/Em = 400/505 nm using a fluorometer or a fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays. Cell Lysis Buffer (Cat.# 1067-100), 2X Reaction Buffer (Cat.# 1068-20), DTT (Cat.# 1201-1), and other reagents used for caspase activity assays are also available separately.

Additional Information

Synonyms	Ac-ATAD-AFC, Caspase-12 Substrate, Fluorogenic
Product #	C-2232
Chemical Name	Ac-ATAD-AFC, Caspase-12 Substrate, Fluorogenic
Formula	C26H30F3N5O10
MW	629.54
Appearance	Liquid
Purity	90% by HPLC
Storage Temp	-20°C

Additional Information

Peptide Sequence	AC-ALA-THR-ALA-ASP-AFC