Description

The NCM356D cell line is an epithelial cell line derived from the normal colon mucosa wide margin resection of a 65-year-old black male with rectal adenocarcinoma (Stauffer et al., 1995). The cells express cytokeratins, villin and other colonic epithelial cell antigens, but are negative for antigens associated with other cell types. Some of the cells are positive for mucin synthesis and a subset of “tumor-associated” antigens (e.g. mutantp53, TAG72). They do not grow in soft agar and are non-tumorigenic. They have fastidious growth requirements and must be maintained in INCELL’s enriched M3:10? medium for long-term in vitro culture with maintenance of an appropriate phenotype