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Concentration
20-50u/μl
5'… GCG↓C…3' 
3'…C↑GCG…5'

Reaction Conditions 
1X Buffer V3 
50mM Tris-HCl (pH7.5 at 30°C), 10mM MgCl₂, 100mM NaCl, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM NaCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
None.

Ligation / Recutting Assay 
After 20-fold overdigestion with AspLEI, more than 90% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 40u of AspLEI for 16 hours at 37°C.
Supplied with 10X Buffer V3, 10X Buffer UB and Viva Buffer A. (Diluent)
*Blocked by CpG methylation

Ordering Information

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Manual
AspLEI {HhaI}