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BmcAI {ScaI} RV1146|产品详情|进口榴莲视频免费观看采购网





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    订购信息
    上海榴莲视频下载地址入口生物科技公司
    Tel:400-968-7988    021-33779008
    BmcAI {ScaI}
    品牌:Vivantis Technologies
    货号:RV1146
    规格:200u
    货期:

    Home Restriction Endonucleases Conventional Restriction Endonucleases BmcAI {ScaI} BmcAI {ScaI} Concentration 1-10u/μl 5'…AGT↓ACT…3' 3'…TCA↑TGA…5'Reaction Conditions 0.5X Buffer UB 12.5mM Tris-acetate (pH 7.6 at 30°C), 5mM Mg-acetate, 50mM K-acetate, 3.5mM 2-mercaptoethanol, and 25μg/ml BSA. Incubate at 37°C.

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    Home  Restriction Endonucleases  Conventional Restriction Endonucleases  BmcAI {ScaI}

    BmcAI {ScaI}

    Concentration
    1-10u/μl 
    5'…AGT↓ACT…3'
    3'…TCA↑TGA…5'

    Reaction Conditions 
    0.5X Buffer UB 

    12.5mM Tris-acetate (pH 7.6 at 30°C), 5mM Mg-acetate, 50mM K-acetate, 3.5mM 2-mercaptoethanol, and 25μg/ml BSA. Incubate at 37°C.

    Storage Buffer
    10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

    Thermal Inactivation
    65°C for 20 minutes.

    Ligation / Recutting Assay 
    After 10-fold overdigestion with BmcAI, 70% of the DNA fragments can be ligated and recut.

    Overdigestion Assay 
    An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of BmcAI for 16 hours at 37°C.
    Supplied with 10X Buffer UB and Viva Buffer A. (Diluent)

    Ordering Information

    Catalog No Description Pack Size
    RV1146 BmcAI {ScaI} 200u

     

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    BmcAI {ScaI}

     
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